What we tested, what we found, what it means and what it does not mean. We would rather be believed than impressive.
MRSA and MDR S. typhi were tested using the same five disc conditions, allowing a direct side-by-side comparison of measured inhibition zones.
Methicillin-resistant Staphylococcus aureus. The chemical comparator discs show little measurable clearance, while the three-compound condition produces the widest zone.
Multidrug-resistant Salmonella enterica serotype Typhi. The same five-condition sequence is shown, with the three-compound condition reaching the largest measured inhibition zone.
Within the same assay, larger zones indicate greater inhibition. Compound identities and exact formulation ratios remain confidential pending patent filing.
Method. Forty-one candidate combinations were screened against clinical MRSA and multidrug-resistant S. typhi. The two chemical comparator conditions used 30 µg; the single, dual and triple plant-derived conditions used 600 µg per component.
Result. The two chemical comparators produced 1–7 mm zones. Activity increased from the single condition to the dual and triple cocktails, reaching 15 mm against MRSA and 20 mm against S. typhi.
Repeated inhibition of two multidrug-resistant clinical isolates justifies the next validation stage.
The funded work must add MIC/MBC, time-kill, resistance development, mechanism, safety, stability, live-bird evidence, independent replication and peer review.
This is the specification a Green Antibiotics product must meet before market entry. Every line is a target with a study attached, not a result already held.
No accumulation in meat or eggs; assessed through residue analysis in the broiler study.
Use through to slaughter; assessed through depletion data and regulatory classification.
Normal health and performance at the intended dose; assessed in safety and tolerance arms.
Seven characterised, shelf-stable cocktails; assessed through MIC panels and stability testing.
Essential-oil compounds can be concentration-dependent and formulation-sensitive.17
Raw plate images, measurement tables, repeat data and the screening protocol are available to investors, partners and scientific collaborators on request.
We publish the denominator, not only the seven leads. A screening claim without its denominator is a marketing claim. This is the standard the founders would apply to a pharmaceutical programme, and the fastest way to judge whether a signal is real.
Alves-Silva J M, Zuzarte M, Girão H, Salgueiro L, et al. Reviews on the antimicrobial efficacy, volatility, instability and concentration-dependent toxicity of essential-oil compounds in animal production, including microencapsulation as a mitigation strategy. Antibiotics, 14(6):552, 2025. mdpi.com/2079-6382/14/6/552